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SRX17033102: GSM6448268: P. donghuensis P482, M9glc Control_3; Pseudomonas donghuensis; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 20.8M spots, 5.8G bases, 1.7Gb downloads

External Id: GSM6448268_r1
Submitted by: Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology UG&MUG, University of Gdansk
Study: Effect of supplementation of M9 0.4% glucose with root exudates of either tomato or maize on gene expression in WT Pseudomonas donghuensis P482
show Abstracthide Abstract
To investigate and compare the influence of root exudates of tomato and maize on Pseudomonas donghuensis P482, we have grown the strain up to a stationary phase in M9 0.4% glucose medium supplemented with maize exudates (Maize), tomato exudates (Tomato) or without supplementation (Control). We then performed differential gene expression analysis, identifying changes in transcriptome profiles between each treatment (Tomato, Maize) and the Control as reference conditions, and between the two treatments. Overall design: Comparative gene expression profiling analysis of RNA-seq data for P482 cells grown in an unsupplemented medium (Control) and in medium supplemented with either tomato exudates (Tomato) or maize exudates (Maize).
Sample: P. donghuensis P482, M9glc Control_3
SAMN30265176 • SRS14617627 • All experiments • All runs
Library:
Name: GSM6448268
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated from cells fixed with fixRNA (Eurx) usingRNeasy Mini Kit (Qiagen). rRNA was depleted with MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific). RNA libraries for RNA-seq were prepared using a Bacterial mRNA prep (according to Baseclear B.V., The Netherlands)
Runs: 1 run, 20.8M spots, 5.8G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR2101661220,810,4555.8G1.7Gb2022-08-15

ID:
23820353

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